Development of the test responds to a call from the World Health Organization (WHO) for a simple, inexpensive method to detect melamine contamination in infant formula and other liquids.

Until now, melamine testing required expensive laboratory equipment and skilled personnel. This kit simplifies the testing and reduces the cost of melamine detection. The MaxDiscovery Melamine Test kit can detect melamine in milk, powdered milk, cream, ice cream and chocolate drink. Bioo Scientific has plans to adapt it to detect melamine in seafood and meat.

Researchers at the BioTechnology Institute (BTI) developed the enzyme, melamine deaminase, used in the MaxDiscovery Melamine Test kit and the enzyme will be produced in the BTI Pilot Plant fermentation facilities. Melamine deaminase works by breaking one of the C-N bonds in melamine to release ammonia, which can be detected by a simple test that turns the liquid blue. Jennifer Seffernick, a research associate in Wackett’s lab, discovered the enzyme while conducting research on biodegradation of s-triazine herbicides. It is one of many examples of how basic research can lead to new technologies that benefit society.

"Development of the melamine enzyme and the test kit is an example of how universities and industry can collaborate to foster basic science, education, and technology that benefits society," says Wackett, who is a Distinguished McKnight University Professor in the College of Biological Sciences.

"Larry Wackett's research has revealed the power of microbial enzymes to modify and destroy toxic substances in the environment," says Joe Krebs, director of Protein Chemistry and Engineering at Bioo Scientific. "Our new enzymatic detection method takes this work in a new direction to provide a better approach for the detection of melamine contamination in the global food supply."

www.sciencedaily.com/releases/2009/07/090721122851.htm